How Many Ounces of Urine is Needed for a Drug Test (4 Tips)

A doctor holding a jar of urine for drug testing

Passing a drug test is a crucial step toward unlocking new employment, scholarship, or insurance opportunities. Understanding the sample requirements is your first step toward achieving these milestones. 

To help you increase your chances of passing, we carefully researched different testing facilities to determine the amount of urine needed for a drug test. Additionally, from our extensive knowledge of drug testing, we outlined the things to avoid before a test and how you can fail one.

Quick Summary

  • The amount of urine needed for a drug test is 30 and 45 milliliters.
  • Drug testing uses urine samples to detect illicit substances in your body. 
  • You can fail a drug test if lab testers detect illegal substances, adulterants, or tampering. 

How Many Ounces of Urine Is Needed for a Drug Test?

A doctor getting a urine sample from a test tubeGenerally, most drug tests need 1-1.5 ounces of urine, equivalent to 30 and 45 milliliters (mL) of urine, or 2-3 tablespoons. However, some tests, like steroids, may require up to 60 milliliters. 

The exact specimen amount may vary depending on the specific testing method used and must be specified by the laboratory. For a 30 ml sample, the laboratory may need approximately 3-5 mL to detect drugs in the body.

Each collection facility has a fixed-size urine testing cup they use to collect urine samples, so it’s important to provide an adequate sample size when submitting a specimen for testing to ensure accurate test results overall; the minimum standard volume of urine needed for drug testing is 30 milliliters. 

“A collector must verify that the specimen contains at least 45 mL of urine.”
US Department of Transport

Also Read: Why is Urine Testing is the Most Popular?

Why You Should Submit the Required Amount?

A person getting samples of urineYou should submit the required amount since urine tests need a sample that increases the accuracy of the results. 

Urine drug tests are designed to detect the presence of specific substances in your urine, such as cocaine, amphetamines, opioids, or THC (the active ingredient in marijuana) and more [1].

These substances appear very small in your urine, so only a small sample is needed to detect them.

Furthermore, providing a larger sample of urine does not necessarily guarantee better accuracy of the test results, so it’s better to stick to the specified amount. 

What You Should Not Do Before a Urine Test? 

A top view of urine sample and other laboratory equipment

You should not do a lot of exercise, eat certain foods, or smoke before a urine test. 

Let’s have an in-depth look below. 

1. Consuming Certain Foods and Drinks

Certain foods and beverages contain chemicals and additives that may appear in your urine sample.

Avoid eating the following foods and drinks before a test to be safe: 

  • Coffee & tea: You should avoid drinking tea at least 72 hours before a urine drug screening as it might appear as metanephrine, indicating a possible withdrawal from alcohol and drugs.  
  • Seafood: Some seafood has arsenic and heavy metals like mercury and lead, which could produce a false positive on a drug test [2]. Avoid eating seafood for at least 48 hours before your drug screening.
  • Acidic fruits, avocados, bananas, and walnuts: Foods like citrus fruits, bananas, avocados, and nuts may also contain high serotonin levels, as found in illicit drugs. These high serotonin levels may appear on a drug test and be mistaken for illegal drug use.
  • Poppy seeds: Eating poppy seed muffins or bagels that drug screening may detect traces of opium, codeine, opium, or morphine, which will lead to a failed test. This is because poppy seeds are coated with opium extract during harvesting [3].  

2. Diluting Urine 

A jar of urine with a blue backgroundThe main indicator of diluted urine is the creatinine concentration of the urine sample.

Creatinine is a byproduct of your muscles’ metabolic process and is excreted by the kidney through urine.

Urine’s creatinine content specifies its concentration as urine concentration increases proportionally with creatinine level.

Urine creatinine levels must be between 20 and 40 mg/dL, while diluted urine has 2 to 20 mg/dL [4]. 

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3. Smoking

You should avoid smoking and second-hand smoking if you’re due for a drug test because passive inhalation may also increase the chances of finding THC in your system. 

4. Avoid Strenuous Exercises 

Exercise burns fat and brings THC back into your body. Avoid any physical activity before the urine drug testing, as this will increase the drug metabolites in the urine.

Additionally, studies have shown that excessive physical activity may increase the appearance of proteins and ketones in urine samples [5]. 

How Can You Fail a Urine Test?

A doctor holding a urine sample while showing a thumbs down

You can fail a urine test if there’s evidence of tampering or adulteration in your urine sample.

Here’s how:

  • Tampering: The major form of tampering with the sample is through dilution. Laboratory tests can detect diluted urine since its creatinine levels and gravity values within the sample are lower than expected for human urine. 
  • Adulteration: An adulterated specimen would cause a urine drug test to be invalid since it alters the pH of the sample. Human urine typically has a pH of around 7, but it can be lower or higher due to certain medications, health issues, or a person’s diet [6]. The urine specimen will be seen as invalid if the pH is outside the range of 3-11, which suggests evidence of tampering.  

Also Read: How Much Visine to Use for a Urine Test


Is 3 Oz of Pee Enough for a Drug Test?

Yes, 3 Oz of pee is enough for a drug test, although it’s way above the minimum requirement of 30 milliliters. Ensure that you follow specimen requirements according to your laboratory to be safe. 

How Many Ounces is a Standard Drug Test?

A standard drug test is 1-1.5 ounces, equivalent to 30-45 millimeters of urine. However, the amount may vary depending on the drug tests performed and the testing facility. 

Is 10 ML Enough for a Urine Test?

No, 10 mL is not enough for a urine test. The minimum amount of urine required is 30 ML. 

How Much Visine to Put In Urine Test?

You should put 100mL of Visine per 1 liter of urine in a urine test. A standard drug test usually requires 30-60mL of urine; therefore, depending on the sample, you must add 3-6mL of Visine.  A study conducted by a laboratory testing an adulterating drug test using Visine found that anything more or less Visine outside of 3-6mL is ineffective [7].

How Much Urine is Needed for a Drug Test

Submitting the required amount of urine for a drug test is essential to ensure compliance with the testing facility and to increase the chances of accuracy.

To achieve this, you must submit a minimum of 30 mL to the testing facility. The required pee amount may vary. That’s why Test Clear Choice Sub Solution provides synthetic pee that caters to changing specimen requirements. 

Test Clear Choice Sub Solution’s synthetic urine is carefully formulated to closely mimic natural human urine, both in chemical composition and appearance. But before using it, be sure to do your research and prioritize your needs. 



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